DEVELOPMENT AND VALIDATION OF NOVEL UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TEPOTINIB IN BULK AND IN PHARMACEUTICAL FORMULATION

Objective: The objective of the study is to develop a new, simple, rapid, accurate, and economical UV-spectrophotometric method validate same for estimation Tepotinib in bulk pharmaceutical formulation as per ICH guidelines. Methods: present work was carried out using UV-Visible double-beam spectrophotometer model Systronics 2201. found soluble water, methanol, methanol so, analytical-grade used solvent conducting work. λmax tepotinib determined by dissolving pure drug scanned range 200-800 nm. validated linearity, accuracy, precision, Limit Detection Quantification. Results: maximum absorbance obtained at wavelength 272 linear 3-15 µg/ml with regression coefficient 0.996 equation y = 0.0699x+0.0335. accuracy be 96.8-98.5%, intra-day inter-day precision % RSD value 0.262 0.69, respectively LOD LOQ were 0.0925 0.28 respectively. Conclusion: demonstrated good reproducibility recovery proposed can successfully applied routine analysis dosage form.